Description

A pyrimidine that is a derivative of cytosine, having a methyl group at the 5-position.

Chemical properties

Chemical formula Net charge Average mass
C5H7N3O 0 125.12860
5-methylcytosine

Recommended notation

Name 5-methylcytosine
Abbreviation 5mC
Symbol m
Complement guanine:5-methylcytosine
Symbol 1

Nomenclature

IUPAC SMILES InChI InChIKey Synonyms
4-amino-5-methylpyrimidin-2(1H)-one Cc1c[nH]c(=O)nc1N InChI=1S/C5H7N3O/c1-3-2-7-5(9)8-4(3)6/h2H,1H3,(H3,6,7,8,9) LRSASMSXMSNRBT-UHFFFAOYSA-N
  • 4-amino-5-methyl-2(1h)-pyrimidinone
  • 4-amino-5-methyl-2-pyrimidinol

Mapping techniques

Method
Method detail
Resolution
Qualifier
References
BS-seq chemical conversion single-base
HELP assay restriction endonuclease low microarray probes
HPoxBS chemical conversion single-base strand-specific
MBD-seq affinity-based low
MRE-seq affinity-based low
Maxam–Gilbert sequencing single-base
MeDIP-seq affinity-based low
Methyl-MAPS affinity-based low
Methyl-seq restriction endonuclease low
MethylCap-seq affinity-based low salt gradient stratification
MspJI recognition restriction endonuclease low specific fragments
PBAT chemical conversion single-base
RRBS chemical conversion single-base methylation-insensitive restriction digestion
SMRT direct detection single-base target sequences
SMRT-BS chemical conversion single-base target sequences
TAPS chemical conversion single-base
TAPSβ chemical conversion single-base
TAmC-seq chemical tagging high
TOP-seq enzyme-mediated chemical tagging high target sequences
Tn5mC-seq chemical conversion single-base
cfMeDIP-seq affinity-based low low-input
coBS-seq chemical conversion single-base
dye-terminator Sanger sequencing direct detection single-base
fluorescence microscopy direct detection low target sequences
lrTAPS chemical conversion single-base
nanopore direct detection single-base target sequences
oxBS-seq chemical conversion single-base

Nature

Origin Function Functional detail
Organisms
References
natural epigenetic mark
  • Arthrobacter luteus
  • Bacillus amyloliquefaciens
  • Bacillus aneurinolyticus
  • Bacillus stearothermophilus
  • Bos taurus
  • Caldicellulosiruptor kristjanssonii
  • Chlamydomonas reinhardtii
  • Desulfotomaculum nigrificans
  • Desulfovibrio desulfuricans
  • Drosophila melanogaster
  • Escherichia coli
  • Fusobacterium nucleatum
  • Haemophilus aegyptius
  • Haemophilus gallinarum
  • Haemophilus haemolyticus
  • Haemophilus parainfluenzae
  • Homo sapiens
  • Klebsiella pneumoniae
  • Locusta migratoria
  • Moraxella nonliquefaciens
  • Mus musculus
  • Nocardia aerocolonigenes
  • Ovis aries
  • Serratia marcescens
  • Streptomyces achromogenes
  • Xanthomonas campestris